2FVZ
Human Inositol Monophosphosphatase 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-5 |
Synchrotron site | MAX II |
Beamline | I911-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9075 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.030, 96.950, 106.970 |
Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.400 |
R-factor | 0.2141 |
Rwork | 0.211 |
R-free | 0.29740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 0.021 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 51914 | |
<I/σ(I)> | 16.85 | 3.2 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 20% PEG 6000, 0.1M Hepes pH 6.8, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |