2FVV

Human Diphosphoinositol polyphosphate phosphohydrolase 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-27
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	48.653, 59.900, 61.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.250
R-factor	0.171
Rwork	0.171
R-free	0.22200
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	0.037
Data reduction software	PROTEUM PLUS
Data scaling software	SADABS
Phasing software	SHELXD
Refinement software	SHELX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.440	1.340
High resolution limit [Å]	1.250	1.250
Rmerge	0.081	0.276
Number of reflections	45852
<I/σ(I)>	23.93	2.74
Completeness [%]	90.5	58.1
Redundancy	8.88	1.55

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	30% PEG8000, 0.2M Lithium sulphate, 0.1M SODIUM ACETATE, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	30% PEG8000, 0.2M Lithium sulphate, 0.1M SODIUM ACETATE, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K