2FV8
The crystal structure of RhoB in the GDP-bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.519, 42.233, 33.850 |
| Unit cell angles | 90.00, 90.58, 90.00 |
Refinement procedure
| Resolution | 34.400 - 1.900 |
| R-factor | 0.21249 |
| Rwork | 0.210 |
| R-free | 0.25324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Swissmodel |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.413 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 14890 | |
| Completeness [%] | 96.7 | 97.5 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 30 % PEG 3350, 0.075M ammonium citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
