2FV7
Crystal structure of human ribokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.530, 72.978, 90.958 |
| Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1RKS 1vm7 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.462 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.046 | 0.024 | 0.261 |
| Number of reflections | 34881 | 3509 | 3403 |
| <I/σ(I)> | 13.6 | ||
| Completeness [%] | 99.3 | ||
| Redundancy | 3 | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 40% PEG-550MME, vapor diffusion, sitting drop, temperature 291K |
