2FV7

Crystal structure of human ribokinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	45.530, 72.978, 90.958
Unit cell angles	90.00, 91.08, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.201
Rwork	0.198
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1RKS 1vm7
RMSD bond length	0.017
RMSD bond angle	1.462
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.046	0.024	0.261
Number of reflections	34881	3509	3403
<I/σ(I)>	13.6
Completeness [%]	99.3
Redundancy	3	2.9	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	40% PEG-550MME, vapor diffusion, sitting drop, temperature 291K