2FUG
Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97564 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.080, 266.112, 201.731 |
| Unit cell angles | 90.00, 104.71, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.265 |
| Rwork | 0.265 |
| R-free | 0.29800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.630 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.988 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.158 | 0.552 |
| Total number of observations | 84269 | |
| Number of reflections | 195669 | 26742 |
| <I/σ(I)> | 6.1 | 1.7 |
| Completeness [%] | 92.7 | 89.2 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
