2FU5
structure of Rab8 in complex with MSS4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.920, 49.850, 83.480 |
| Unit cell angles | 102.88, 97.46, 90.12 |
Refinement procedure
| Resolution | 19.170 - 2.000 |
| R-factor | 0.19964 |
| Rwork | 0.197 |
| R-free | 0.25213 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hxr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.325 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.170 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 40718 | |
| Completeness [%] | 94.5 | 74.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 20% PEG3350, 50mM TrisHCL pH 9.0, 0.2M magnesium acetate, 0.15% (w/v) 1,2,3-heptanetriole, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
