2FTY
Crystal structure of dihydropyrimidinase from Saccharomyces kluyveri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.428, 73.320, 162.181 |
| Unit cell angles | 90.00, 91.59, 90.00 |
Refinement procedure
| Resolution | 43.850 - 2.400 |
| R-factor | 0.21996 |
| Rwork | 0.217 |
| R-free | 0.27370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nfg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.193 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 83629 | |
| <I/σ(I)> | 13.5 | 3.2 |
| Completeness [%] | 99.3 | 98.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.1M ammonium sulfate, 0.001mM zinc chloride, 0.1M bis-tris , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
