2FTP
Crystal Structure of hydroxymethylglutaryl-CoA lyase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 189.012, 189.012, 189.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.000 - 2.400 |
| R-factor | 0.18799 |
| Rwork | 0.187 |
| R-free | 0.21309 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ydn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.432 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.086 | 0.530 |
| Number of reflections | 21999 | |
| <I/σ(I)> | 12.7 | 9.97 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 42.6 | 42.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris 8.0, 3.4M Sodium Formate, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
