2FT3
Crystal structure of the biglycan dimer core protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-06-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1159 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.511, 119.222, 140.625 |
| Unit cell angles | 90.00, 116.61, 90.00 |
Refinement procedure
| Resolution | 23.000 - 3.400 |
| R-factor | 0.259 |
| Rwork | 0.258 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xku |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.276 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.900 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 40497 | |
| Completeness [%] | 96.5 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M triammonium citrate, 20% (v/v) polyethylene glycol 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
