2FRS
Crystal structure of the f15w mutant of apo-cellular retinoic acid binding protein type ii at 1.51 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2003-03-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.582, 37.030, 58.693 |
| Unit cell angles | 102.00, 106.12, 93.00 |
Refinement procedure
| Resolution | 33.900 - 1.510 |
| R-factor | 0.18 |
| Rwork | 0.174 |
| R-free | 0.24000 |
| Structure solution method | Rigid body refinement |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.593 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.560 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.032 | 0.261 |
| Number of reflections | 39496 | 3188 |
| <I/σ(I)> | 14.38 | 1.69 |
| Completeness [%] | 92.8 | 75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 30% (w/v) PEG 4000, 0.2 M Bis-Tris-Propane pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
