2FP4
Crystal structure of pig GTP-specific succinyl-CoA synthetase in complex with GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.11588 |
Spacegroup name | P 65 |
Unit cell lengths | 132.610, 132.610, 72.530 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 116.038 - 2.080 |
R-factor | 0.214 |
Rwork | 0.195 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.900 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 116.038 | 72.990 | 2.210 |
High resolution limit [Å] | 2.080 | 6.640 | 2.100 |
Rmerge | 0.055 | 0.019 | 0.362 |
Total number of observations | 8067 | 22254 | |
Number of reflections | 43486 | 1442 | 6210 |
<I/σ(I)> | 11.9 | 28.6 | 2 |
Completeness [%] | 99.3 | ||
Redundancy | 5.5 | 5.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 5 mM GTP, 10 mM MgCl2, 20% w/v polylethylene glycol 3350, 150 mM KF, VAPOR DIFFUSION, HANGING DROP, temperature 294K |