2FMX
An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-11 |
| Detector | ENRAF-NONIUS GX-21 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.825, 61.993, 71.126 |
| Unit cell angles | 90.00, 107.58, 90.00 |
Refinement procedure
| Resolution | 39.070 - 1.820 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.070 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.091 | 0.411 |
| Number of reflections | 35092 | |
| <I/σ(I)> | 11.8 | 2 |
| Completeness [%] | 88.6 | 63.8 |
| Redundancy | 3.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 298 | 28%(w/v)Polyethylene Glycol-4000, 100mM sodium acetate, 0.2M ammonium sulfate, pH 5.6, EVAPORATION, temperature 298K |
