2FMM

Crystal Structure of EMSY-HP1 complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-24
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97860, 0.97910, 0.96500
Spacegroup name	P 21 21 2
Unit cell lengths	143.400, 63.870, 66.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.219
Rwork	0.219
R-free	0.24300
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.052	0.220
Number of reflections	54308
<I/σ(I)>	15.8
Completeness [%]	94.4	96
Redundancy	5.4	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	1.8M ammonium sulfate, 0.1M tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K