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2FMD

Structural basis of carbohydrate recognition by Bowringia milbraedii seed agglutinin

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsEMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron siteEMBL/DESY, HAMBURG
BeamlineX11
Temperature [K]100
Detector technologyCCD
Collection date2005-06-08
DetectorMARRESEARCH
Wavelength(s)0.8123
Spacegroup nameI 2 2 2
Unit cell lengths66.603, 86.351, 91.760
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution15.000 - 1.900
R-factor0.186
Rwork0.183
R-free0.22800
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1q8s CHAIN A
RMSD bond length0.008
RMSD bond angle1.674
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASER
Refinement softwareCNS (1.1)
Data quality characteristics
 Overall
Low resolution limit [Å]15.000
High resolution limit [Å]1.900
Rmerge0.095
Number of reflections18493
Completeness [%]87.3
Redundancy5.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.527715% PEG 20000, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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