2FLP
Binary complex of the catalytic core of human DNA polymerase iota with DNA (template G)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 98.367, 98.367, 202.497 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.510 - 2.400 |
R-factor | 0.2328 |
Rwork | 0.228 |
R-free | 0.27616 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB CODE ALZ |
RMSD bond length | 0.060 |
RMSD bond angle | 3.182 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.055 |
Number of reflections | 22540 |
<I/σ(I)> | 16.5 |
Completeness [%] | 98.3 |
Redundancy | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | pH 6.5, VAPOR DIFFUSION, HANGING DROP |