2FLL
Ternary complex of human DNA polymerase iota with DNA and dTTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.329, 98.329, 202.186 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.22765 |
| Rwork | 0.223 |
| R-free | 0.27747 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 2AZL |
| RMSD bond length | 0.043 |
| RMSD bond angle | 2.929 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.059 |
| Number of reflections | 18496 |
| <I/σ(I)> | 34.5 |
| Completeness [%] | 99.7 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | pH 6.5, VAPOR DIFFUSION, HANGING DROP |
