2FLI
The crystal structure of D-ribulose 5-phosphate 3-epimerase from Streptococus pyogenes complexed with D-xylitol 5-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.022, 199.449, 87.622 |
| Unit cell angles | 90.00, 109.76, 90.00 |
Refinement procedure
| Resolution | 24.990 - 1.800 |
| R-factor | 0.227 |
| Rwork | 0.224 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tqj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.990 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 229414 | |
| Completeness [%] | 94.9 | 88.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 19% PEG 3350, 0.1M Succinic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
