2FID
Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2820 |
| Spacegroup name | P 61 |
| Unit cell lengths | 81.967, 81.967, 54.443 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.070 - 2.800 |
| R-factor | 0.21687 |
| Rwork | 0.211 |
| R-free | 0.26775 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.070 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 5005 | |
| Completeness [%] | 95.7 | 77.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 20-25% PEG 4000, 0.2M lithium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
