2FIB
RECOMBINANT HUMAN GAMMA-FIBRINOGEN CARBOXYL TERMINAL FRAGMENT (RESIDUES 143-411) COMPLEXED TO THE PEPTIDE GLY-PRO-ARG-PRO AT PH 6.0
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.330, 68.200, 47.600 |
| Unit cell angles | 90.00, 105.02, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.010 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.28200 |
| Structure solution method | ISOMORPHOUS WITH PDB ENTRY 1FIB |
| Starting model (for MR) | 1fib |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.430 * |
| Data reduction software | RIGAKU (SOFTWARE) |
| Data scaling software | RIGAKU |
| Phasing software | X-PLOR (3.1) |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.200 | 2.250 |
| High resolution limit [Å] | 2.000 | 2.010 |
| Rmerge | 0.054 | |
| Number of reflections | 12275 | |
| <I/σ(I)> | 11 | 3.17 |
| Completeness [%] | 76.9 | 61.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | PROTEIN WAS CRYSTALLIZED FROM 12% PEG8000, 70MM CACL2, 0.1 M MES, PH 6.0, 0.02%NAN3, ROOM TEMPERATURE, SITTING DROPS. THE CRYSTAL WAS SOAKED OVERNIGHT IN THE ORIGINAL SOLUTION PLUS 0.01M GLY-PRO-ARG-PRO (SIGMA), THEN BACK-SOAKED FOR 10 MINUTES., vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 12 (%) | |
| 2 | 1 | reservoir | 70 (mM) | ||
| 3 | 1 | reservoir | MES | 0.1 (M) | |
| 4 | 1 | reservoir | 0.02 (%) | ||
| 5 | 1 | drop | protain | 15 (mg/ml) | |
| 6 | 1 | drop | PEG8000 | 6 (%) | |
| 7 | 1 | drop | 35 (mM) | ||
| 8 | 1 | drop | MES | 0.05 (M) | |
| 9 | 1 | drop | 0.01 (%) |
