2FH7
Crystal structure of the phosphatase domains of human PTP SIGMA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97931 |
Spacegroup name | P 61 |
Unit cell lengths | 94.333, 94.333, 123.036 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.670 - 2.000 |
R-factor | 0.19199 |
Rwork | 0.190 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lar |
RMSD bond length | 0.009 |
RMSD bond angle | 1.148 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.760 | 1.910 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.093 | 0.011 |
Number of reflections | 55608 | |
<I/σ(I)> | 6 | 0.6 |
Completeness [%] | 99.0 | 93.3 |
Redundancy | 9.3 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | Protein(10mg/ml) was crystallized in (15% PEG3350,100mM Succinic Acid)., pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |