2FGI
CRYSTAL STRUCTURE OF THE TYROSINE KINASE DOMAIN OF FGF RECEPTOR 1 IN COMPLEX WITH INHIBITOR PD173074
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08-27 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 208.790, 57.380, 66.180 |
Unit cell angles | 90.00, 107.55, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.500 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.26400 |
RMSD bond length | 0.009 |
RMSD bond angle | 22.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.816) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.500 | |
Rmerge | 0.047 | 0.252 * |
Total number of observations | 93535 * | |
Number of reflections | 24664 | |
<I/σ(I)> | 12.4 | |
Completeness [%] | 94.5 | 95.5 * |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 4 * | Mohammadi, M., (1996) Cell, 86, 577. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-50 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | dithiothreitol | 2 (mM) | |
5 | 1 | reservoir | PEG10000 | 16 (%) | |
6 | 1 | reservoir | ammonium sulfate | 0.3 (M) | |
7 | 1 | reservoir | ethylene glycol | 5 (%) | |
8 | 1 | reservoir | bis-Tris | 100 (mM) |