2FG7
N-succinyl-L-ornithine transcarbamylase from B. fragilis complexed with carbamoyl phosphate and N-succinyl-L-norvaline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 43 |
| Unit cell lengths | 156.424, 156.424, 120.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.900 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fg6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.154 | 0.871 |
| Number of reflections | 64660 | |
| <I/σ(I)> | 9 | 1.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 56% tascimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
