2FC0
WRN exonuclease, Mn dGMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.104, 81.104, 93.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.900 - 2.000 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fbt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.085 |
| Number of reflections | 24008 | |
| <I/σ(I)> | 54.2 | 8.5 |
| Completeness [%] | 98.1 | 93.4 |
| Redundancy | 6.75 | 5.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 277 | 5ul of 4.5mg/ml WRN exonuclease buffered in 25mM Tris HCl, 100mM NaCl, pH 7.5, mixed with 5ul 1% MPEG 2K, 200mM Na Acetate, pH 4.5 from the reservior solution and 1ul EDTA additive, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 4.50 |
