2FBW

Avian respiratory complex II with carboxin bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9800
Spacegroup name	P 1 21 1
Unit cell lengths	118.700, 200.753, 67.631
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	64.090 - 2.060
R-factor	0.1728
Rwork	0.171
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yq3
RMSD bond length	0.007
RMSD bond angle	0.862
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX (dev_3150)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.090	2.120
High resolution limit [Å]	2.060	2.060
Rmerge	0.192	0.630
Rmeas	0.216	0.754
Rpim	0.097	0.401
Number of reflections	167170	8064
<I/σ(I)>	6	2
Completeness [%]	85.3	49.4
Redundancy	4.19	2.99
CC(1/2)	0.959	0.103

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	50 G/L PEG-3350, 25 ML/L ISOPROPANOL, 15 ML/L PEG-400 0.05 M NA-HEPES, 0.01 M TRIS-HCL, 0.0005 M MNCL2, 0.0013 M MGCL2, 0.0015 M NA-AZIDE, 0.00025 M NA-EDTA, CARBOXIN, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 277K