2FAH
The structure of mitochondrial PEPCK, Complex with Mn and GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.595, 90.852, 103.339 |
| Unit cell angles | 64.23, 73.74, 71.18 |
Refinement procedure
| Resolution | 32.810 - 2.090 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.23366 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2FAG |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.305 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.180 |
| High resolution limit [Å] | 2.090 | 4.520 | 2.090 |
| Rmerge | 0.106 | 0.045 | 0.581 |
| Number of reflections | 127447 | ||
| Completeness [%] | 95.3 | 98.8 | 91.9 |
| Redundancy | 3.3 | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 16% PEG6000, 0.1M HEPES, n-octanoylsucrose, GDP, MnCl2, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
