2FAF
The structure of chicken mitochondrial PEPCK.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.807, 47.737, 127.205 |
| Unit cell angles | 90.00, 111.31, 90.00 |
Refinement procedure
| Resolution | 34.080 - 1.700 |
| R-factor | 0.162 |
| Rwork | 0.161 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1khg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.332 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.054 | 0.034 | 0.541 |
| Number of reflections | 123916 | ||
| Completeness [%] | 90.9 | 99 | 86.3 |
| Redundancy | 4.3 | 5.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 19% PEG 6000, HEPES pH 7.4, n-octanoyl sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
