2F9J

3.0 angstrom resolution structure of a Y22M mutant of the spliceosomal protein p14 bound to a region of SF3b155

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-21
Detector	ADSC QUANTUM 210
Spacegroup name	P 41
Unit cell lengths	71.271, 71.271, 102.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.250 - 3.000
R-factor	0.22128
Rwork	0.219
R-free	0.26586
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 2f9d
RMSD bond length	0.010
RMSD bond angle	1.328
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.059	0.023	0.460
Number of reflections	10288
Completeness [%]	99.8	98.2	100
Redundancy	4.2	4.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	60% NaH2PO4/40% K2HPO4 0.5M beta-alanine, VAPOR DIFFUSION, HANGING DROP, temperature 298K