2F8Y

Crystal structure of human Notch1 ankyrin repeats to 1.55A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Detector technology	CCD
Collection date	2005-01-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9791
Spacegroup name	P 65
Unit cell lengths	97.933, 97.933, 109.068
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.550
R-factor	0.152
Rwork	0.150
R-free	0.18700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ot8
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	70977
<I/σ(I)>	11.9	2
Completeness [%]	82.7	45.9
Redundancy	2.2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1