2F8V
Structure of full length telethonin in complex with the N-terminus of titin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8117 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.513, 46.885, 128.033 |
| Unit cell angles | 90.00, 98.30, 90.00 |
Refinement procedure
| Resolution | 23.900 - 2.750 |
| R-factor | 0.26734 |
| Rwork | 0.267 |
| R-free | 0.28733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ya5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.403 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.900 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.101 | |
| Number of reflections | 33692 | |
| Completeness [%] | 96.1 | 79.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.45 | 298 | PEG35000, LITHIUM SULPHATE, ACETATE BUFFER, pH 4.45, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
