2F8F
Crystal structure of the Y10F mutant of the gluathione s-transferase from schistosoma haematobium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.227, 77.594, 53.143 |
| Unit cell angles | 90.00, 94.26, 90.00 |
Refinement procedure
| Resolution | 53.450 - 2.100 |
| R-factor | 0.22423 |
| Rwork | 0.221 |
| R-free | 0.28751 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1OE7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.245 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.452 | 2.154 |
| High resolution limit [Å] | 1.993 | 2.100 |
| Number of reflections | 23175 | |
| Completeness [%] | 96.6 | 98.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | ammonium sulphate 2.1 M, 100 mM tris, 5mM b-mercaptoethanol, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
