2F7T
Crystal structure of the catalytic domain of Mos1 mariner transposase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97624 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 44.243, 44.243, 206.047 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.250 |
R-factor | 0.19345 |
Rwork | 0.189 |
R-free | 0.27685 |
Structure solution method | MAD |
RMSD bond length | 0.025 |
RMSD bond angle | 2.017 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.350 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.130 | |
Number of reflections | 10257 | |
<I/σ(I)> | 13 | 6.3 |
Completeness [%] | 97.1 | 85.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 24-26% PEG 4000, 0.1M Tris, 0.005M MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 24-26% PEG 4000, 0.1M Tris, 0.005M MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |