2F6C

Reaction geometry and thermostability of pyranose 2-oxidase from the white-rot fungus Peniophora sp., Thermostability mutant E542K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-14
Detector	MARRESEARCH
Wavelength(s)	0.8123
Spacegroup name	P 42 21 2
Unit cell lengths	101.931, 101.931, 118.602
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.700 - 1.840
R-factor	0.15184
Rwork	0.150
R-free	0.18640
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.015
RMSD bond angle	1.456
Data scaling software	APRV
Phasing software	EPMR
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.000	1.880
High resolution limit [Å]	1.840	1.840
Number of reflections	54750
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	100 mM MES/NaOH, pH=6.0, 19% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 293K