2F5X
Structure of periplasmic binding protein BugD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-08 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.670, 76.420, 260.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.630 - 1.720 |
R-factor | 0.14 |
Rwork | 0.140 |
R-free | 0.17500 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.187 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.630 | 1.810 |
High resolution limit [Å] | 1.720 | 1.720 |
Number of reflections | 101925 | |
<I/σ(I)> | 7.1 | 3 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 10.9 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 278 | Tris 10 mM, (NH4)2SO4 0.2 M, PEG1500 36% (w/v), glycerol 15%, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 278K |