2F57

Crystal Structure Of The Human P21-Activated Kinase 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9788
Spacegroup name	C 1 2 1
Unit cell lengths	98.661, 56.599, 120.764
Unit cell angles	90.00, 103.13, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.15525
Rwork	0.154
R-free	0.18513
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 2C30
RMSD bond length	0.010
RMSD bond angle	1.226
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	60040
Completeness [%]	99.4	94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	0.2M Na/KPO4, 0.1M BTProp 7.5, 20% PEG3350, 10% Ethelene glycol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K