2F4W
Human ubiquitin-conjugating enzyme E2 J2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-04 |
| Detector | SBC-3 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.070, 48.014, 91.032 |
| Unit cell angles | 90.00, 93.94, 90.00 |
Refinement procedure
| Resolution | 30.840 - 2.000 |
| R-factor | 0.18962 |
| Rwork | 0.186 |
| R-free | 0.25015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ayz |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.642 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.840 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.238 |
| Number of reflections | 23045 | |
| <I/σ(I)> | 23.67 | 4.74 |
| Completeness [%] | 95.8 | 85.6 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 298 | VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
