2F4U

Asite RNA + designer antibiotic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Collection date	2002-06-01
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	33.100, 46.460, 89.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.230 - 2.600
R-factor	0.299
Rwork	0.250
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	d*TREK
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.230	23.230	2.690
High resolution limit [Å]	2.600	5.580	2.600
Rmerge	0.092		0.423
Number of reflections	4451
<I/σ(I)>	5.8		1.6
Completeness [%]	97.6	93.2	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1