2F2L
Crystal structure of tracheal cytotoxin (TCT) bound to the ectodomain complex of peptidoglycan recognition proteins LCa (PGRP-LCa) and LCx (PGRP-LCx)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2005-10-22 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.123, 79.695, 114.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.16872 |
Rwork | 0.166 |
R-free | 0.21378 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb entries 1S2J and 1Z6I |
RMSD bond length | 0.010 |
RMSD bond angle | 1.329 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.088 | 0.497 |
Number of reflections | 23701 | |
<I/σ(I)> | 2 | |
Completeness [%] | 98.0 | 90.2 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.2 | 298 | 20% polyethylene glycol 1500, 5% ethylene glycol, and 100 mM phosphate-citrate, pH 4.2, EVAPORATION, temperature 298.0K |