2F1X
Crystal structure of the TRAF-like domain of HAUSP/USP7 bound to a p53 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.756, 39.560, 101.885 |
Unit cell angles | 90.00, 105.32, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 2F1W |
RMSD bond length | 0.007 |
RMSD bond angle | 1.520 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 15182 | |
Completeness [%] | 92.2 | 73.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG2000 monomethylether, calcium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |