2F1D
X-Ray Structure of imidazoleglycerol-phosphate dehydratase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 157.949, 157.949, 479.965 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.24262 |
| Rwork | 0.240 |
| R-free | 0.28633 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rhy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.277 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.097 |
| Number of reflections | 88754 |
| <I/σ(I)> | 11.7 |
| Completeness [%] | 98.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
