2F0A
Crystal Structure of Monomeric Uncomplexed form of Xenopus dishevelled PDZ domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798, 0.9801, 0.9611 |
| Spacegroup name | P 61 |
| Unit cell lengths | 89.834, 89.834, 82.471 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.450 - 1.800 |
| R-factor | 0.21571 |
| Rwork | 0.214 |
| R-free | 0.25263 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.696 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.450 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 34969 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 297 | 1.4 M ammonium sulfate 0.1 M sodium cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
