2F02
Crystal Structure of LacC from Enterococcus Faecalis in complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | .9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.463, 62.739, 93.454 |
Unit cell angles | 90.00, 99.91, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.20308 |
Rwork | 0.201 |
R-free | 0.23996 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.180 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0016) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.065 | 0.281 |
Number of reflections | 57959 | |
<I/σ(I)> | 11.2 | 2.37 |
Completeness [%] | 98.0 | 96.2 |
Redundancy | 3.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.4M NACITRATE, 0.1M TRIS PH 8.5, 0.01M ATP, CRYO-XYLITOL, VAPOR DIFFUSION, HANGING DROP, temperature 293K |