2EZ7
Carbonic anhydrase activators. Activation of isozymes I, II, IV, VA, VII and XIV with L- and D-histidine and crystallographic analysis of their adducts with isoform II: engineering proton transfer processes within the active site of an enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.060, 41.430, 72.520 |
| Unit cell angles | 90.00, 104.40, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.29 |
| Rwork | 0.249 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.340 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.520 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 15977 | |
| Completeness [%] | 84.0 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 275 | 2.4 M ammonium sulphate, tris, HCl 50 mM, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 275K |
