2EYQ
Crystal structure of Escherichia coli transcription-repair coupling factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2004-07-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98166 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.870, 161.990, 161.730 |
Unit cell angles | 90.00, 105.09, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.200 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.29500 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 3.200 |
Number of reflections | 61409 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 28% pentaerythritol ethoxylate 100 mM HEPES, pH 7.5 75mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |