2EXU

Crystal Structure of Saccharomyces cerevisiae transcription elongation factors Spt4-Spt5NGN domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-03
Detector	MARRESEARCH
Wavelength(s)	1.2834
Spacegroup name	P 41 2 2
Unit cell lengths	53.801, 53.801, 177.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.420 - 2.230
R-factor	0.207
Rwork	0.206
R-free	0.22100
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.994
Data scaling software	SCALA
Phasing software	SOLVE (2.09)
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.840	2.330
High resolution limit [Å]	2.220	2.230
Rmerge	0.088	0.262
Number of reflections	13576
<I/σ(I)>	5.4	2.8
Completeness [%]	99.7	99.7
Redundancy	7.7	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	30% EtOH, 50mM NaCl, 100mM Tris-HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	30% EtOH, 50mM NaCl, 100mM Tris-HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	30% EtOH, 50mM NaCl, 100mM Tris-HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K