2EXE
Crystal structure of the phosphorylated CLK3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97640 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.180, 110.998, 161.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.820 - 2.350 |
| R-factor | 0.22653 |
| Rwork | 0.225 |
| R-free | 0.26028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z57 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.235 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.820 | |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.050 | 0.343 |
| Number of reflections | 23665 | |
| <I/σ(I)> | 13.59 | |
| Completeness [%] | 99.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1.8M (NH4)3(cit), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
