2ETX

Crystal Structure of MDC1 Tandem BRCT Domains

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Wavelength(s)	0.97954, 0.97967, 0.98120
Spacegroup name	P 1
Unit cell lengths	41.890, 44.350, 63.100
Unit cell angles	70.98, 86.45, 61.91

Refinement procedure

Resolution	59.340 - 1.330
R-factor	0.20279
Rwork	0.202
R-free	0.22574
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.296
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.840	1.364
High resolution limit [Å]	1.330	1.330
Number of reflections	85639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	MES 0.1M, PEG 8000 15%, ETHYLENE GLYCOL 15%, AMMONIUM ACETATE 0.2M, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K