2ES0
Structure of the regulator of G-protein signaling domain of RGS6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 77.567, 77.567, 73.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 67.120 - 2.100 |
R-factor | 0.19636 |
Rwork | 0.195 |
R-free | 0.22855 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2A72.pdb |
RMSD bond length | 0.008 |
RMSD bond angle | 0.979 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.930 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.083 | 0.011 |
Number of reflections | 15210 | |
<I/σ(I)> | 15.4 | 1.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.1 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 10K; (NH4)(ac); BIS-TRIS , pH pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |