2ES0

Structure of the regulator of G-protein signaling domain of RGS6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	77.567, 77.567, 73.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.120 - 2.100
R-factor	0.19636
Rwork	0.195
R-free	0.22855
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2A72.pdb
RMSD bond length	0.008
RMSD bond angle	0.979
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.930	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.083	0.011
Number of reflections	15210
<I/σ(I)>	15.4	1.7
Completeness [%]	99.9	100
Redundancy	7.1	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 10K; (NH4)(ac); BIS-TRIS , pH pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K