2ERX
Crystal Structure of DiRas2 in Complex With GDP and Inorganic Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98400 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.041, 74.931, 85.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.320 - 1.650 |
| R-factor | 0.16288 |
| Rwork | 0.162 |
| R-free | 0.18797 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1KAO |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.729 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.320 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.241 | 0.232 |
| Number of reflections | 42390 | |
| Completeness [%] | 99.3 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG 3350; 0.20M Sodium Chloride; 0.1M BIS-TRIS, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
