2ERC
CRYSTAL STRUCTURE OF ERMC' A RRNA-METHYL TRANSFERASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 1996-10 |
| Detector | TATE ET AL. (1995) J. APPL. CRYST. 28, 196-205. |
| Spacegroup name | P 6 |
| Unit cell lengths | 146.900, 146.900, 57.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.030 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.31300 |
| Structure solution method | MAD PHASING IN A DIFFERENT EXPERIMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 21.850 * |
| Data reduction software | HKL ((DENZO)) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.500 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.068 | 0.027 |
| Total number of observations | 138382 * | |
| Number of reflections | 14568 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 94.0 | 84.3 |
| Redundancy | 9.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | pH 4.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8-10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | drop | SAH | 40-fold molar excess | |
| 7 | 1 | reservoir | ammonium sulfate | 3.2 (M) | |
| 8 | 1 | reservoir | citrate | 0.1 (M) |
