2ER8
Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 94 |
| Detector technology | CCD |
| Collection date | 2002-08-10 |
| Wavelength(s) | 1.2830, 1.2833, 1.2587 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 107.500, 107.500, 218.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.530 - 2.850 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.26500 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 33071 | |
| <I/σ(I)> | 40.9 | 4.2 |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM Hepes, 22% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Hepes | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | Hepes | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | H2O |
