2ER8
Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 94 |
Detector technology | CCD |
Collection date | 2002-08-10 |
Wavelength(s) | 1.2830, 1.2833, 1.2587 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 107.500, 107.500, 218.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.530 - 2.850 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.26500 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 33071 | |
<I/σ(I)> | 40.9 | 4.2 |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM Hepes, 22% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Hepes | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | H2O | ||
4 | 1 | 2 | Hepes | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | H2O |